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This combination of multiplexed biomarker measurements and subcellular spatial resolution enables segmentation of the image into individual cells, making possible subsequent cell type classification and quantification.

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T-cell response was determined using flow cytometry following hour co-culture with tumor cells.

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Multiplex immunostainings allow studying the interaction of different cell types in the tumor microenvironment using a single tissue slide.

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We foresee this technique to greatly facilitates the execution of high-plex stainings and thereby the discovery of novel tumor-microenvironment interactions.

Description: Multiple preclinical studies and clinical trials have attempted to target these cells; however, monitoring responses to these therapies remains a challenge.

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